Ghosh, Kalyan Sundar ; Maiti, Tushar Kanti ; Mandal, Abhishek ; Dasgupta, Swagata (2006) Copper complexes of (−)-epicatechin gallate and (−)-epigallocatechin gallate act as inhibitors of Ribonuclease A Federation of European Biochemical Societies Letters, 580 (19). pp. 4703-4708. ISSN 0014-5793
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Official URL: https://www.sciencedirect.com/science/article/pii/...
Related URL: http://dx.doi.org/10.1016/j.febslet.2006.07.054
Abstract
Green tea polyphenols, which have the ability to inhibit angiogenesis, form complexes with Cu(II), a known potent stimulator of blood vessel proliferation. Copper complexes of (−)-epicatechin gallate and (−)-epigallocatechin gallate were found to inhibit the enzymatic activity of Ribonuclease A (RNase A) as revealed by an agarose gel based assay and urea denatured gel electrophoresis. The copper complexes were found to be non-competitive inhibitors of RNase A with inhibition constants in the micromolar range. Changes in the secondary structure of the protein are found to occur due to the interaction as revealed from Fourier transform infrared and circular dichroism studies.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Copper Complexes; Green Tea Polyphenols; Ribonuclease A; Noncompetitive Inhibition; Fourier Transformed Infrared; Circular Dichroism |
| ID Code: | 113759 |
| Deposited On: | 15 May 2018 06:23 |
| Last Modified: | 15 May 2018 06:23 |
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