Ghosh, Sudeshna ; Pandey, Nitin K. ; Bhattacharya, Susmita ; Roy, Anushree ; Nagy, Nóra Veronika ; Dasgupta, Swagata (2015) Evidence of two oxidation states of copper during aggregation of hen egg white lysozyme (HEWL) International Journal of Biological Macromolecules, 76 . pp. 1-9. ISSN 0141-8130
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Official URL: https://www.sciencedirect.com/science/article/pii/...
Related URL: http://dx.doi.org/10.1016/j.ijbiomac.2015.02.014
Abstract
In vitro fibrillation of hen egg white lysozyme (HEWL) causes complete reduction of Cu(II) to Cu(I) at pH 7. Here in the present article, we have shown the presence of both Cu(II) and Cu(I) at pH 11 during fibrillation of HEWL using electron paramagnetic resonance and Raman spectroscopy. Our results suggest the existence of a partially reducing environment during fibrillation of hen egg white lysozyme at pH 11. The fibrillation process is governed by the pH of the solution and maximum fibrillation is found to occur at pH 11. Fibrils formed in the absence of Cu(II) were also found to cause significant hemolysis of RBC.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Hen Egg White Lysozyme; Fibrillation; Cu(II); Oxidation State; Hemolysis |
| ID Code: | 113502 |
| Deposited On: | 15 May 2018 06:16 |
| Last Modified: | 15 May 2018 06:16 |
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