Vinay Kumar, N. ; Bernstein, Lori R. (2001) A new analytical scale DNA affinity binding assay for analyses of specific protein–DNA interactions Analytical Biochemistry, 299 (2). pp. 203-210. ISSN 0003-2697
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Official URL: https://www.sciencedirect.com/science/article/pii/...
Related URL: http://dx.doi.org/10.1006/abio.2001.5433
Abstract
We describe a rapid analytical assay for identification of proteins binding to specific DNA sequences. The DAPSTER assay (DNA affinity preincubation specificity test of recognition assay) is a DNA affinity chromatography-based microassay that can discriminate between specific and nonspecific protein–DNA interactions. The assay is sensitive and can detect protein–DNA interactions and larger multicomponent complexes that can be missed by other analytical methods. Here we describe in detail the optimization and utilization of the DAPSTER assay to isolate AP-1 complexes and associated proteins in multimeric complexes bound to the AP-1 DNA element.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 113479 |
| Deposited On: | 25 May 2018 05:51 |
| Last Modified: | 25 May 2018 05:51 |
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