Wavelength dependent, sequentially activated, dual anticancer drug delivery system with photoinduced fluorescence off-on for real time imaging

Abstract

Development of new photoresponsive drug delivery systems for combination chemotherapy is sought‐after due to their precise spatio‐temporal control over delivering drugs. Further, design of wavelength dependent drug delivery systems with each individual drug release being “one on one” triggered by light of different activating wavelengths, is an emerging topic for its high selective control over the release of individual drug. Herein we are reporting design of a new wavelength dependent photoresponsive dual drug delivery system i. e. nitric oxide donor linked coumarin chlorambucil conjugate (NOD‐Cou‐Cbl) for sequentially delivering anticancer agents with orthogonal mode of actions. NOD‐Cou‐Cbl has shown a sequential release of nitric oxide (NO) followed by chlorambucil under step wise irradiation with visible light and UV light, respectively. We have also studied in vitro cytotoxicity activity and real time bioimaging property of dual DDS on MDA‐MB‐231 cell line.