Axial resolution limit of a fiber-optic fluorescence probe

Balaji, J. ; Garai, Kanchan ; Chakrabarti, Subhasis ; Maiti, Sudipta (2003) Axial resolution limit of a fiber-optic fluorescence probe Applied Optics, 42 (19). pp. 3780-3784. ISSN 0003-6935

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Official URL: https://www.osapublishing.org/ao/abstract.cfm?uri=...

Related URL: http://dx.doi.org/10.1364/AO.42.003780

Abstract

We examine the limit of spatial resolution achievable when a single optical fiber is used for excitation and collection of fluorescence from a bulk specimen. We calculate the probability of detecting a fluorescent particle as a function of its position relative to the fiber face, using excitation wavelength λ, radius a, numerical aperture N.A., and the particle’s fluorescence and absorbance spectra. Treating Rhodamine B as a model fluorescent analyte and using appropriate fiber parameters, we show that the maximum axial resolution (defined as the axial distance in a homogenous solution within which 50% of the detected signal originates) achievable is ∼10 μm. We experimentally measured the axial resolution for a 500-μM aqueous solution of Rhodamine B with λ = 543 nm, a = 1.31 μm, and a N.A. of 0.16 and found good qualitative agreement with the calculation.

Item Type:Article
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ID Code:113083
Deposited On:24 May 2018 09:22
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