Kaushalya, S. K. ; Nag, Suman ; Balaji, J. ; Maiti, S. (2008) Serotonin: Multiphoton imaging and relevant spectral data In: SPIE BiOS, San Jose, California, United States.
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Official URL: https://www.spiedigitallibrary.org/conference-proc...
Related URL: http://dx.doi.org/10.1117/12.763020
Abstract
Coupling three-photon microscopy with automated stage movement can now produce a live high resolution map of the neurotransmitter serotonin in a single cross section of the whole rat brain. Accurate quantification of these serotonin images demands appropriate spectral filtering. This requires one to consider that the spectral characteristics of serotonin show a remarkable variation as it non-covalently associates with different molecules, as we discuss here. Also it is known that serotonin emission changes when it forms a covalent adduct with para-formaldehyde. This provides a potential route for producing a whole brain serotonin map using multiphoton microscopy in a fixed rat brain. Here we take the initial step showing that multiphoton microscopy of this adduct can quantitatively image chemically induced changes in serotonin distribution.
Item Type: | Conference or Workshop Item (Paper) |
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Source: | Copyright of this article belongs to SPIE. |
Keywords: | Luminescence; Brain; Multiphoton microscopy; Tissues; Molecules; Brain Mapping; Spectroscopy; Stars; Neurotransmitters; Microscopy |
ID Code: | 113070 |
Deposited On: | 25 May 2018 04:16 |
Last Modified: | 25 May 2018 04:16 |
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