Sengupta, Parijat ; Balaji, J. ; Maiti, Sudipta (2002) Measuring diffusion in cell membranes by fluorescence correlation spectroscopy Methods, 27 (4). pp. 374-387. ISSN 1046-2023
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Official URL: https://www.sciencedirect.com/science/article/pii/...
Related URL: http://dx.doi.org/10.1016/S1046-2023(02)00096-8
Abstract
Fluorescence Correlation Spectroscopy (FCS) can measure diffusion on the cell surface with unparalleled sensitivity. In appropriate situations, this can be the most sensitive and accurate method for measuring receptor interaction and oligomerization. Here we attempt to describe FCS in sufficient detail so that the reader is able to judge when there is a compelling reason to choose this technique, understand the basic theory behind it, construct a FCS spectrometer in the laboratory, and analyze the data to obtain a meaningful estimate of the physical parameters.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 112914 |
| Deposited On: | 24 May 2018 09:04 |
| Last Modified: | 24 May 2018 09:04 |
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