Diller, Rolf ; Iannone, Mark ; Cowen, Benjamin R. ; Maiti, Sudipta ; Bogomolni, Roberto A. ; Hochstrasser, Robin M. (1992) Picosecond dynamics of bacteriorhodopsin, probed by time-resolved infrared spectroscopy Biochemistry, 31 (24). pp. 5567-5572. ISSN 0006-2960
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Official URL: https://pubs.acs.org/doi/10.1021/bi00139a020
Related URL: http://dx.doi.org/10.1021/bi00139a020
Abstract
The photoinduced reaction cycle of bacteriorhodopsin (BR) has been studied by means of a recently developed picosecond infrared spectroscopic method at ambient temperature. BR - K difference spectra between 1560 and 1700 cm-1 have been recorded at delay times from 100 ps to 14 ns. The spectrum remains unchanged during this period. The negative difference OD band at 1660 cm-1 indicates the peptide backbone responds within 50 ps. A survey in the region of carboxylic side chain absorption around 1740 cm-1 reveals that perturbations of those groups, present in low-temperature FTIR spectra, are not observable within 10 ns, suggesting a slow conformational change.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to American Chemical Society. |
| ID Code: | 112912 |
| Deposited On: | 24 May 2018 11:48 |
| Last Modified: | 24 May 2018 11:48 |
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