MYC2 differentially regulates GATA-box conaining promoters during seedling development in Arabidopsis

Abstract

MYC2 is an important transcription factor, which modulates transcription by directly binding to Z-, G- and E-box elements present in the promoters of light and different stress responsive genes. Very recently, we have shown that MYC2 plays a role in the regulation of Z- and/or G-box containing promoters during both seedling and adult plant growth. Although, MYC2 does not bind to the GATA box light responsive element (LRE) in vitro as shown in DNA binding assays, its involvement in the regulation of GATA-box containing promoter in planta, if any, is not known. Here, we report that the promoter activity of GATA-NOS101 in atmyc2 mutant was found to be similar to wild-type in BL and dark grown seedlings, whereas it was found to be lower compared with wild-type as revealed from GUS staining results. Further, we will discuss the consequences of MYC2 regulating GATA-box containing promoter in combination with G-box containing promoters.