Kumar, Vinod ; Shorie, Munish ; Ganguli, Ashok K. ; Sabherwal, Priyanka (2015) Graphene-CNT nanohybrid aptasensor for label free detection of cardiac biomarker myoglobin Biosensors and Bioelectronics, 72 . pp. 56-60. ISSN 0956-5663
Full text not available from this repository.
Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.bios.2015.04.089
Abstract
We report a label free electrochemical detection of cardiac bio-marker myoglobin (Mb) on aptamer functionalized rGO/CNT nanostructured electrodes by measuring its direct electron transfer (DET). Configured as a highly responsive aptasensor, the newly developed biosensing platform exhibits synergistic effect of the nano-hybrid functional construct by combining good electrical properties and the facile chemical functionality of nanohybrid for the compatible bio-interface development. The specific anti-Mb aptamer was generated by five iterative SELEX (Systematic evolution of ligands by exponential enrichment) rounds, showing high senstivity (KD ~65 pM). The aptamer functionalized rGO/CNT nanostructured electrodes demonstrated a significant increase in signal response with a detection limit of ~0.34 ng/mL in the dynamic response range between 1 ng/mL and 4 µg/mL for Mb. The newly developed DET assay format presents a promising candidate in point-of-care diagnosis for routine screening of Mb in patient's samples.
Item Type: | Article |
---|---|
Source: | Copyright of this article belongs to Elsevier Science. |
Keywords: | Cardiac Marker; Myoglobin; Aptamer; rGO/CNT Nanohybrid; Direct Electron Transfer |
ID Code: | 111621 |
Deposited On: | 26 Sep 2017 13:11 |
Last Modified: | 26 Sep 2017 13:11 |
Repository Staff Only: item control page