Inducible reactivation of UV-irradiated cholera phage e5 in Vibrio cholerae MAK757

Basu, Rina ; Ghosh, Amit (1987) Inducible reactivation of UV-irradiated cholera phage e5 in Vibrio cholerae MAK757 Molecular and General Genetics MGG, 209 (1). pp. 175-178. ISSN 0026-8925

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Official URL: http://www.springerlink.com/content/n2wl7626t0n524...

Related URL: http://dx.doi.org/10.1007/BF00329855

Abstract

The survival of UV-irradiated cholera phage e5 was found to increase when the host cells, Vibrio cholerae MAK757, were exposed to a low dose of UV irradiation before phage infection (Weigle reactivation), indicating the existence of a UV-inducible DNA repair pathway (SOS repair) in V. cholerae MAK757. The induction signal generated by UV irradiation was transient in nature and lasted about 20-30 min at 37° C. Maximal weigle reactivation of the phage was obtained when the host cells were irradiated with a UV dose of 16 J/m2. V. cholerae MAK757 was also found to possess efficient photoreactivation and host cell reactivation of UV-damaged DNA in phage e5.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Inducible Reactivation; Cholera Phage; SOS
ID Code:11105
Deposited On:08 Nov 2010 06:54
Last Modified:11 Feb 2011 11:50

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