Effect of acriflavine on ultraviolet inactivation of Acholeplasma laidlawii

Ghosh, Amit ; Das, Jyotirmoy ; Maniloff, Jack (1978) Effect of acriflavine on ultraviolet inactivation of Acholeplasma laidlawii Biochimica et Biophysica Acta (BBA) - General Subjects, 543 (4). pp. 570-575. ISSN 0304-4165

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/030441...

Related URL: http://dx.doi.org/10.1016/0304-4165(78)90311-2

Abstract

An increased sensitivity to inactivation was observed when ultraviolet light-irradiated Acholeplasma laidlawii An increase sensitivity to inactivation was observed when ultraviolet light-irradiated Acholeplasma laidlawii cells were plated on medium containing either acriflavine or chloramphenicol. Chloramphenicol reduced liquid holding recovery (dark repair) to about 10 percent of that in untreated irradiated cells. In acriflavine treated cells no dark repair could be observed and there was a progressive degradation of cell DNA during holding. While the primary effect of acriflavine may be to inhibit excision repair, since ultraviolet-irradiated Mycoplasma gallisepticum (cells which lack an excision repair mechanism) show a slight increase in inactivation when plated on medium containing acriflavine, the dye must also have some other effects on ultraviolet repair processes. Acriflavine treatment of A. laidlawii cells before ultraviolet irradiation has a protective effect, as seen by an increased cell survival.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:11100
Deposited On:09 Nov 2010 03:59
Last Modified:16 Feb 2011 04:46

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