Bhattacharyya, Riddhi ; Das, Krishna ; Sen, Sandeep ; Roy, Syamal ; Majumder, Hemanta K. (1996) Development of a genus specific primer set for detection of leishmaniaparasites by polymerase chain reaction FEMS Microbiology Letters, 135 (2-3). pp. 195-200. ISSN 0378-1097
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Official URL: https://academic.oup.com/femsle/article-lookup/doi...
Related URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb07989.x
Abstract
We have compared the sequences of a major class of kinetoplast DNA (kDNA) minicircle (pLURkE3) of Leishmania strain UR6 with other minicircle sequences from different Leishmaniaspecies. Alignment of these sequences allowed the selection of a pair of oligonucleotides suitable as primers in polymerase chain reaction (PCR) which is specific for Leishmaniaparasites. PCR with this genus-specific primer set is capable of detecting 1 femtogram of kDNA. These primers have been tested with kDNAs from both old world and new world Leishmaniaspecies. The results indicate that the primers may be suitable for detection of any kind of leishmaniasis.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons, Inc |
| Keywords: | Leishmaniaspecies; Genus-specific Primer; Polymerase Chain Reaction |
| ID Code: | 110506 |
| Deposited On: | 31 Jan 2018 09:41 |
| Last Modified: | 31 Jan 2018 09:41 |
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