A simple and rapid liquid chromatography method for simultaneous determination of zidovudine and nevirapine in plasma

Ramachandran, Geetha ; Hemanthkumar, A. K. ; Kumaraswami, V. ; Swaminathan, Soumya (2006) A simple and rapid liquid chromatography method for simultaneous determination of zidovudine and nevirapine in plasma Journal of Chromatography B, 843 (2). pp. 339-344. ISSN 1570-0232

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.jchromb.2006.06.014

Abstract

We describe a simple, fast, isocratic, reversed-phase high performance liquid chromatographic method for simultaneous determination of plasma zidovudine and nevirapine with UV detection at 260 nm. The method involves liquid–liquid extraction with ethyl acetate and using 3-isobutyl 1-methyl xanthine as internal standard. The system requires a C18 column (150 mm × 4.6 mm I.D.) and a mobile phase composed of potassium dihydrogen phosphate (15 mM; pH 7.5) and acetonitrile in the ratio of 80:20 (v/v). The assay was linear from 0.025 to 10.0 μg/ml for zidovudine and 0.05 to 10.0 μg/ml for nevirapine. The intra- and inter-day variations were less than 10% for both the drugs. The method was specific and sensitive enough to allow quantification of zidovudine and nevirapine in concentrations observed clinically. The average recoveries of zidovudine and nevirapine from plasma were 95 and 94%, respectively. The method was applied to a pharmacokinetic study in HIV-infected patients who were receiving antiretroviral treatment with zidovudine and nevirapine containing regimens. The method spans the blood concentration range of clinical interest. Due to its simplicity, the assay can be used for pharmacokinetic studies and therapeutic drug monitoring in patients taking a combination treatment of zidovudine and nevirapine.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Zidovudine; Nevirapine; Plasma; HPLC
ID Code:109948
Deposited On:01 Sep 2017 09:13
Last Modified:01 Sep 2017 09:13

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