Mitra, Sreyoshi ; Rai, Laxmi Shanker ; Chatterjee, Gautam ; Sanyal, Kaustuv (2016) Chromatin immunoprecipitation (ChIP) assay in Candida albicans Methods in Molecular Biology, 1356 . pp. 43-57. ISSN 1064-3745
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Official URL: https://link.springer.com/protocol/10.1007%2F978-1...
Related URL: http://dx.doi.org/10.1007/978-1-4939-3052-4_4
Abstract
Chromatin immunoprecipitation (ChIP) is a widely used technique which can determine the in vivo association of a specific protein on a particular DNA locus in the genome. In this method cross-linked chromatin is sheared and immunoprecipitated with antibodies raised against a target protein of interest. The end result of this process is the enrichment of DNA fragments associated with the desired protein. Thus, interactions between proteins and genomic loci in cellular context can be determined by this technique. Here, we are describing a ChIP protocol that is optimized for Candida albicans. The protocol requires 4–5 days for completion of the assay and has been used to produce robust ChIP results for diverse proteins in this organism and its related species including Candida dubliniensis and Candida tropicalis.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer Verlag. |
| Keywords: | Protein–DNA Interaction; Antibody; Affinity Purification; Candida Dubliniensis; Candida Tropicalis; Polymerase Chain Reaction (PCR); Microarray; Next-Generation Sequencing |
| ID Code: | 109842 |
| Deposited On: | 25 Oct 2017 13:09 |
| Last Modified: | 25 Oct 2017 13:09 |
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