Barak, Pradeep ; Rai, Ashim ; Rai, Priyanka ; Mallik, Roop (2013) Quantitative optical trapping on single organelles in cell extract Nature Methods, 10 (1). pp. 68-70. ISSN 1548-7091
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Official URL: http://www.nature.com/nmeth/journal/v10/n1/full/nm...
Related URL: http://dx.doi.org/10.1038/nmeth.2287
Abstract
We have developed an optical trapping method to precisely measure the force generated by motor proteins on single organelles of unknown size in cell extract. This approach, termed VMatch, permits the functional interrogation of native motor complexes. We apply VMatch to measure the force, number and activity of kinesin-1 on motile lipid droplets isolated from the liver of normally fed and food-deprived rats.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Nature Publishing Group. |
| Keywords: | Cell Biology; Optical Tweezers; Single-molecule Biophysics |
| ID Code: | 109326 |
| Deposited On: | 10 Oct 2017 13:22 |
| Last Modified: | 10 Oct 2017 13:22 |
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