Shetty, Deeti K. ; Shetty, Ronak ; Inamdar, Maneesha S. (2016) Generation of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-On Stem Cell Research, 16 (2). pp. 246-248. ISSN 1873-5061
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.scr.2015.12.045
Abstract
Human embryonic stem cell line BJNhem20-OCIAD1-Tet-On was generated using non-viral method. The constructs pCAG-Tet-On and pTRE-Tight vector driving OCIAD1 expression were transfected using microporation procedure. pCAG-Tet-On cells can be used for inducible expression of any coding sequence cloned into pTRE-Tight vector. For example, in human embryonic stem cells, Tet-On system has been used to generate SOX2 overexpression cell line (Adachi et al., 2010).
| Item Type: | Article |
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| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 109191 |
| Deposited On: | 01 Feb 2018 04:55 |
| Last Modified: | 01 Feb 2018 04:55 |
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