Talha, Sheikh M. ; Juntunen, Etvi ; Salminen, Teppo ; Sangha, Amninder ; Vuorinen, Tytti ; Khanna, Navin ; Pettersson, Kim (2015) All-in-one dry-reagent time-resolved immunofluorometric assay for the rapid detection of HIV-1 and -2 infections Journal of Virological Methods, 226 . pp. 52-59. ISSN 0166-0934
Full text not available from this repository.
Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.jviromet.2015.10.004
Abstract
An all-in-one (AIO) dry-reagent time-resolved fluorometric immunoassay that requires minimal liquid handling was developed for the detection of anti-HIV-1 and -2 antibodies. To prepare the AIO wells, in vivo biotinylated capture antigens (r-Bio-HIV-1env and r-Bio-HIV-2env) were immobilized on streptavidin-coated microtitration wells and Eu (III) chelate labelled non-biotinylated tracer antigens [r-HIV-1env-Eu (III) and r-HIV-2env-Eu (III)] were dried in stable form in the same wells. The HIV AIO assay was evaluated with serum/plasma samples (n = 148) from in-house and commercial panels at two different incubation times of 15 min and 1 h. The overall sensitivity of the AIO assay was 98.6% and specificity was 100% for both the incubation times. The AIO assay can accept whole blood matrix. This assay is envisioned to fill the gap between the rapid point-of-care assays and traditional Enzyme Immunoassays (EIA) in terms of complexity and turnaround time, without compromising the performance.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | All-in-one; Time-resolved Fluorometry; Immunoassay; HIV; Diagnostics; Europium; HIV Antibody; Testing |
| ID Code: | 109114 |
| Deposited On: | 09 Mar 2018 12:12 |
| Last Modified: | 09 Mar 2018 12:12 |
Repository Staff Only: item control page
