Myyrylainen, Tiina ; Talha, Sheikh M. ; Swaminathan, Sathyamangalam ; Vainionpaa, Raija ; Soukka, Tero ; Khanna, Navin ; Pettersson, Kim (2010) Simultaneous detection of Human Immunodeficiency Virus 1 and Hepatitis B virus infections using a dual-label time-resolved fluorometric assay Journal of Nanobiotechnology, 8 . Article ID 27. ISSN 1477-3155
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Official URL: https://jnanobiotechnology.biomedcentral.com/artic...
Related URL: http://dx.doi.org/10.1186/1477-3155-8-27
Abstract
A highly specific and novel dual-label time-resolved immunofluorometric assay was developed exploiting the unique emission wavelengths of the intrinsically fluorescent terbium (Tb3+) and europium (Eu3+) tracers for the simultaneous detection of human immunodeficiency virus 1 (HIV-1) and Hepatitis B Virus (HBV) infections, respectively. HIV-1 infection was detected using a double antigen sandwich format wherein anti-HIV-1 antibodies were captured using an in vivo biotinylated version of a chimeric HIV-1 antigen and revealed using the same antigen labeled with Tb3+ chelate. Hepatitis B surface antigen (HBsAg), which served as the marker of HBV infection, was detected in a double antibody sandwich using two monoclonal antibodies (mAbs), one chemically biotinylated to capture, and the other labeled with Eu3+ nanoparticles, to reveal. The performance of the assay was evaluated using a collection (n = 60) of in-house and commercially available human sera panels. This evaluation showed the dual-label assay to possess high degrees of specificity and sensitivity, comparable to those of commercially available, single analyte-specific kits for the detection of HBsAg antigen and anti-HIV antibodies. This work demonstrates the feasibility of developing a potentially time- and resource-saving multiplex assay for screening serum samples for multiple infections in a blood bank setting.
Item Type: | Article |
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Source: | Copyright of this article belongs to BioMed Central. |
ID Code: | 109059 |
Deposited On: | 09 Mar 2018 12:10 |
Last Modified: | 09 Mar 2018 12:10 |
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