An integrin-targeting RGDK-tagged nanocarrier: anticancer efficacy of loaded curcumin

Das, Krishnendu ; Nimushakavi, Sahithi ; Chaudhuri, Arabinda ; Das, Prasanta Kumar (2017) An integrin-targeting RGDK-tagged nanocarrier: anticancer efficacy of loaded curcumin ChemMedChem, 12 (10). pp. 738-750. ISSN 1860-7179

Full text not available from this repository.

Official URL: http://onlinelibrary.wiley.com/doi/10.1002/cmdc.20...

Related URL: http://dx.doi.org/10.1002/cmdc.201700085

Abstract

Herein we report the design and development of α5β1 integrin-specific noncovalent RGDK–lipopeptide-functionalized single-walled carbon nanotubes (SWNTs) that selectively deliver the anticancer drug curcumin to tumor cells. RGDK tetrapeptide-tagged amphiphiles were synthesized that efficiently disperse SWNTs with a suspension stability index of >80 % in cell culture media. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)- and lactate dehydrogenase (LDH)-based cell viability assays in tumor (B16F10 melanoma) and noncancerous (NIH3T3 mouse fibroblast) cells revealed the non-cytotoxic nature of these RGDK–lipopeptide–SWNT conjugates. Cellular uptake experiments with monoclonal antibodies against αvβ3, αvβ5, and α5β1 integrins showed that these SWNT nanovectors deliver their cargo (Cy3-labeled oligonucleotides, Cy3-oligo) to B16F10 cells selectively via α5β1 integrin. Notably, the nanovectors failed to deliver the Cy3-oligo to NIH3T3 cells. The RGDK–SWNT is capable of delivering the anticancer drug curcumin to B16F10 cells more efficiently than NIH3T3 cells, leading to selective killing of B16F10 cells. Results of Annexin V binding based flow cytometry experiments are consistent with selective killing of tumor cells through the late apoptotic pathway. Biodistribution studies in melanoma (B16F10)-bearing C57BL/6J mice showed tumor-selective accumulation of curcumin intravenously administered via RGDK–lipopeptide–SWNT nanovectors.

Item Type:Article
Source:Copyright of this article belongs to John Wiley & Sons, Inc.
ID Code:108545
Deposited On:01 Feb 2018 11:14
Last Modified:01 Feb 2018 11:14

Repository Staff Only: item control page