Datta, Karabi ; Potrykus, Ingo ; Datta, Swapan K. (1992) Efficient fertile plant regeneration from protoplasts of the Indica rice breeding line IR72 (Oryza sativa L.) Plant Cell Reports, 11 (5-6). pp. 229-233. ISSN 0721-7714
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Official URL: https://link.springer.com/article/10.1007/BF002350...
Related URL: http://dx.doi.org/10.1007/BF00235071
Abstract
Protoplasts were isolated from embryogenic cell suspensions obtained from mature seed derived embryogenic callus of the advanced Indica type rice breeding line IR72 available from IRRI (International Rice Research Institute), Manila. Culture of protoplasts with the agarose bead type method without nurse culture led to sustained proliferation of protoplast derived clones. A simple culture protocol was developed which stimulated embryogenic development. Germination of somatic embryos has so far produced 277 green plants from 6 independent experiments. 117 plants have been transferred to soil and are growing in the greenhouse. A few of them have already flowered and set seeds.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer Verlag. |
| ID Code: | 108517 |
| Deposited On: | 01 Feb 2018 11:10 |
| Last Modified: | 01 Feb 2018 11:10 |
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