Datta, Karabi ; Tu, Jumin ; Oliva, Norman ; Ona, Isabelita ; Velazhahan, Rethinasamy ; Mew, Twng Wah ; Muthukrishnan, Subbaratnam ; Datta, Swapan K. (2001) Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars Plant Science, 160 (3). pp. 405-414. ISSN 0168-9452
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/S0168-9452(00)00413-1
Abstract
Genetic transformation has been attempted for management of rice sheath blight disease, caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system. Inheritance was studied up to the T2 generation by Southern blot analysis. Western blot analysis of transgenic plants with polyclonal antibody revealed the presence of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase antibody. The transformants synthesized different levels of chitinase proteins constitutively and progeny from the plants containing the chitinase gene showed different levels of enhanced resistance when challenged with the sheath blight pathogen R. solani.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Sheath Blight; Chitinase; Transgenic Rice; Enhanced Resistance |
| ID Code: | 108424 |
| Deposited On: | 01 Feb 2018 11:08 |
| Last Modified: | 01 Feb 2018 11:08 |
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