Barawkar, D. A. ; Kumar, V. A. ; Ganesh, K. N. (1994) Triplex formation at physiological pH by oligonucleotides incorporating 5-Me-dC-(N4-spermine) Biochemical and Biophysical Research Communications, 205 (3). pp. 1665-1670. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...
Related URL: http://dx.doi.org/10.1006/bbrc.1994.2859
Abstract
Oligonucleotide (ODN) directed tripler formation has therapeutic importance and depends on Hoogsteen hydrogen bonds between a duplex DNA and a third strand. While T∗A:T triplets are formed at neutral pH, C+∗G:C are favoured at acidic pH. Herein it is shown that 18-mer ODN containing spermine conjugated to 5-Me-dC at N4 (1-5), form triplexes with complementary 24-mer duplex 8:9 at neutral pH (7.3, 100 mM NaCl). Under such conditions, control ODN's carrying dC (6) or 5-Me-dC (7) did not show any triple helix formation. Remarkably, the triplexes from spermine-conjugates (1-5) have foremost stability at neutral pH (7.1), unlike the behavior of normal ODN's where optimal stability is at acidic pH (5.5). These results have importance in designing oligonucleotides for antigene applications.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 10823 |
| Deposited On: | 09 Nov 2010 04:46 |
| Last Modified: | 31 May 2011 09:55 |
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