Gopalakrishnan, Vidhya ; Ghadage, R. S. ; Ganesh, K. N. (1991) Influence of internucleotide phosphate linkage on relative base stacking in 3'-5' and 2'-5' RNA: a circular dichroic spectroscopic study of RNA hexamer AACCUU Biochemical and Biophysical Research Communications, 180 (3). pp. 1251-1257. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...
Related URL: http://dx.doi.org/10.1016/S0006-291X(05)81330-0
Abstract
The variations in base stacking interactions of two isomeric RNA hexamers, 3'-5'r(AACCUU) and 2'-5'r'(AACCUU), have been studied using temperature dependent CD spectroscopy. Both RNA hexamers, in single strand form, exhibited a right handed helical sense. Van't Hoff analysis of the CD spectral results, derived from a two state model, gave a higher enthalpy of stacking for 3'-5' RNA than for 2'-5'RNA. The results suggest that 3'-5' linkage in RNA facilitates formation of better helical stacks in relation to an isomeric 2'-5' linkage.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 10786 |
| Deposited On: | 09 Nov 2010 04:54 |
| Last Modified: | 31 May 2011 10:04 |
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