S12. 10 role of mitochondrial DNA mutations in periodontitis

Abstract

Periodontitis is an inflammatory disease affecting the tissues that surround the teeth, as a result of complex interactions between pathogenic bacteria and the host’s immune response. There are increasing evidences on the implications of Reactive Oxygen Species (ROS) in the pathogenesis of inflammatory disorders. Therefore, we aimed to analyze the role of genetics, biochemical, membrane potential and evolutionary background in periodontitis. We have examined a total of 92 patients, of which, 30 (Chronic Periodontitis [CP] group) were selected for the above analysis. The institutional ethics committee approved this study and informed written consent was obtained from each participant. All clinical parameter scores of the tissue sampling areas in the CP group were significantly higher (< 0.001) than in the control group. Complete mtDNA sequencing revealed a total of 264 variations in the patients, including 16 novel mutations, of which 3 were missense mutations (A4234T, A7796G and G8115R). We also observed significant change in the membrane potential and protein levels of NADH dehydrogenase, Cytochrome c oxidase and HSP 60 in the patients.