Effect of preculturing conditions on growth of Lactobacillus rhamnosus on medium containing glucose and citrate

Jyoti, B. D. ; Suresh, A. K. ; Venkatesh, K. V. (2004) Effect of preculturing conditions on growth of Lactobacillus rhamnosus on medium containing glucose and citrate Microbiological Research, 159 (1). pp. 35-42. ISSN 0944-5013

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.micres.2004.01.008

Abstract

Lactobacillus rhamnosus can metabolize citrate through a citrate inducible transport system. The growth curves of L. rhamnosus on medium containing glucose and citrate was found to be highly dependent on preculturing conditions. It exhibited diauxic growth when precultured on glucose, but demonstrated simultaneous consumption when cultured on citrate. The maximum specific growth rate for cells growing on glucose+citrate was 0.38 h−1, which was higher than the growth rate on individual substrates (0.28 h−1). Simultaneous consumption also yielded higher net flavour compounds, diacetyl and acetoin. Flux analysis indicated that L. rhamnosus requires oxygen for balancing excess NADH through NADH oxidase. The flux analysis provided insights into the metabolic network of L. rhamnosus.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Flux Analysis; Lactobacillus; Multiple Substrates; Preculturing; Microaerophilic
ID Code:107305
Deposited On:20 Jun 2017 11:47
Last Modified:20 Jun 2017 11:47

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