Gupta, Gagan D. ; Makde, Ravindra D. ; Kamdar, Radhika P. ; D‧Souza, Jacinta S. ; Kulkarni, Meeta G. ; Kumar, Vinay ; Rao, Basuthkar J. (2005) Co-expressed recombinant human translin-trax complex binds DNA FEBS Letters, 579 (14). pp. 3141-3146. ISSN 0014-5793
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Official URL: http://onlinelibrary.wiley.com/doi/10.1016/j.febsl...
Related URL: http://dx.doi.org/10.1016/j.febslet.2005.05.007
Abstract
Trax, expressed alone aggregates into insoluble complexes, whereas upon co-expression with Translin becomes readily soluble and forms a stable heteromeric complex (∼430 kDa) containing both proteins at nearly equimolar ratio. Based on the subunit molecular weights, estimated by MALDI-TOF-MS, the purified complex appears to comprise of either an octameric Translin plus a hexameric Trax (calculated MW 420 kDa) or a heptamer each of Trax and Translin (calculated MW 425 kDa) or a hexameric Translin plus an octameric Trax (calculated MW 431 kDa). The complex binds single-stranded/double-stranded DNA. ssDNA gel-shifted complex shows both proteins at nearly equimolar ratio, suggesting that Translin "chaperones" Trax and forms heteromeric complex that is DNA binding competent.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons Inc. |
| ID Code: | 107196 |
| Deposited On: | 16 Jun 2017 07:44 |
| Last Modified: | 16 Jun 2017 07:45 |
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