Singh, Himanshu ; Raghavan, Vandana ; Shukla, Manish ; Rao, Basuthkar J. ; Chary, Kandala V. R. (2014) 1H,13C and 15N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii Biomolecular NMR Assignments, 8 (1). pp. 71-74. ISSN 1874-2718
Full text not available from this repository.
Official URL: http://link.springer.com/article/10.1007%2Fs12104-...
Related URL: http://dx.doi.org/10.1007/s12104-012-9455-1
Abstract
Almost complete sequence specific 1H, 13C and 15N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii are reported. The cDNA of S114A mutant of UVI31+ was cloned from a eukaryotic green algae (C. reinhardtii) and overexpressed in E.coli from where the protein was purified to homogeneity. The point mutation S114A in UVI31+ reduces its DNA endonuclease activity substantially as compared with its wild type. As a prelude to the structural characterization of S114A mutant of UVI31+, we report here complete sequence-specific 1H, 13C and 15N NMR assignments.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer Verlag. |
| Keywords: | NMR; Resonance Assignments; S114A; UVI31+; Chlamydomonas reinhardtii |
| ID Code: | 106741 |
| Deposited On: | 16 Jun 2017 11:00 |
| Last Modified: | 16 Jun 2017 11:00 |
Repository Staff Only: item control page
