Dash, Kalpana ; Saha, K. ; Gangal, S. V. (1993) Natural serum haemagglutinins (lectins) in catfish Clarias batrachus: purification and binding studies with rabbit erythrocytes Fish and Shellfish Immunology, 3 (5). pp. 361-370. ISSN 1050-4648
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S10504...
Related URL: http://dx.doi.org/10.1006/fsim.1993.1035
Abstract
A natural haemagglutinin against rabbit erythrocytes has been purified from sera of Clarias batrachus, an Indian fresh water catfish, using ammonium sulphate fractionation followed by Sephadex G-200 chromatography and high performance liquid chromatography. The haemagglutinin has a molecular weight of about 200 kDa is composed of two subunits having molecular weights of 64 and 68 kDa. Thus presumably it has three chains bound by non-covalent bonds. The number of binding sites for this haemagglutinin on a single rabbit erythrocyte is 3.46 × 106. This binding is a spontaneous reaction (KA = 2.80 × 107 l mol-1; ΔF= -1.0515 × 104 kcal mol-1).
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Purification; Catfish; Serum; Haemagglutinins |
| ID Code: | 10652 |
| Deposited On: | 04 Nov 2010 06:46 |
| Last Modified: | 01 Jun 2011 11:49 |
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