Tanuja, S. ; Srinivas, N. D. ; Raghava Rao, K. S. M. S. ; Gowthaman, M. K. (1997) Aqueous two-phase extraction for downstream processing of amyloglucosidase Process Biochemistry, 32 (8). pp. 635-641. ISSN 1359-5113
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/S0032-9592(97)00009-5
Abstract
A polymer/salt aqueous two-phase system has been successfully employed for separation and purification of amyloglucosidase. The effects of system pH, molecular weight of polymer and composition of the two-phase system on amyloglucosidase partition behaviour in polyethylene glycol (PEG 4000, 6000)/disodium hydrogen phosphate were investigated. Experimental data are explained based on Kim's theoretical model for the prediction of biomolecule partitioning in a PEG/salt system.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 105564 |
| Deposited On: | 21 Dec 2017 11:52 |
| Last Modified: | 21 Dec 2017 11:52 |
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