Chethana, S. ; Rastogi, N. K. ; Raghavarao, K. S. M. S. (2006) New aqueous two phase system comprising polyethylene glycol and xanthan Biotechnology Letters, 28 (1). pp. 25-28. ISSN 0141-5492
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Official URL: http://link.springer.com/article/10.1007/s10529-00...
Related URL: http://dx.doi.org/10.1007/s10529-005-4682-5
Abstract
A new aqueous two phase system comprising polyethylene glycol and xanthan is reported together with its phase diagram. The phase composition of the bottom phase did not vary (PEG 1.6–1.8% w/w; xanthan 0.24–0.28% w/w) while that of the top phase varied significantly (PEG 4–5% w/w, xanthan 0.05–1.37% w/w). Unlike conventional aqueous two phase systems, the viscosity of the top phase is also high and values are comparable to that of the bottom phase. When BSA was used as a model protein, it partitioned entirely into the bottom phase.
Item Type: | Article |
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Source: | Copyright of this article belongs to Springer Verlag. |
Keywords: | Aqueous Two Phase System; Partition Coefficient; Phase Diagram; Proteins; Xanthan |
ID Code: | 105486 |
Deposited On: | 21 Dec 2017 11:49 |
Last Modified: | 21 Dec 2017 11:49 |
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