Chethana, S. ; Patil, Ganapathi ; Madhusudhan, M. C. ; Raghavarao, K. S. M. S. (2008) Electrophoretic extraction of cells/particles in a counter current extractor Separation Science and Technology, 43 (14). pp. 3583-3600. ISSN 0149-6395
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Official URL: http://www.tandfonline.com/doi/full/10.1080/014963...
Related URL: http://dx.doi.org/10.1080/01496390802212708
Abstract
Electrophoretic separation is a leading method for resolving mixtures of charged macromolecules or cells. In the present work, a combination of free electrophoresis and multistage extraction called Electrophoretic Counter Current Extractor was explored. A simple mathematical model was developed to describe the mass and heat transfer during the electrophoretic separation. In order to validate the model and also to test the extractor, the extraction of fixed human Red Blood Cells and Latex particles of different sizes suspended in phosphate buffer, was performed at different electric field strengths. The experimental results were found to agree reasonably well with those predicted by the model.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Taylor & Francis. |
| Keywords: | Counter Current; Electrophoresis; Electrophoretic Mobility; Extraction; Separation |
| ID Code: | 104296 |
| Deposited On: | 21 Dec 2017 11:56 |
| Last Modified: | 21 Dec 2017 11:56 |
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