Paenibacillus arachidis sp. nov., isolated from groundnut seeds

Abstract

A Gram-stain-positive, endospore-forming, rod-shaped, facultatively anaerobic bacterium, designated as strain E3^T, was isolated from groundnut seeds. Based on the 16S rRNA gene sequence analysis, strain E3^T belongs to the genus Paenibacillus with P aenibacillus thailandensis S3-4A^T (96.0 %), Paenibacillus xanthinilyticus 11N27^T (95.7 %), Paenibacillus mendelii C/2^T (95.7 %) and other members of the genus Paenibacillus (<95.5 %) as its closest phylogenetic neighbours. The DNA G+C content of strain E3^T was 53 mol%. Strain E3^T was positive for gelatin hydrolysis, ammonification, catalase, chitinase production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, HCN production, siderophore production, biofilm formation, and urea and starch hydrolysis. Strain E3^T had phosphatidylethanolamine, diphosphotidylglycerol, phosphatidylcholine, an unidentified aminophospholipid, two unidentified aminolipids and two unidentified lipids as polar lipids. Strain E3^T had diploptene, deplopterol and bacteriohopaneterol as major hopanoids. anteiso-C_15 : 0 was the predominant cellular fatty acid with significant proportions of iso-C_16 : 0, C_16 : 0, C_17 : 0, anteiso-C_17 : 0, C_18 : 1ω9c and iso-C_14 : 0. Strain E3^T had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. On the basis of physiological, biochemical, chemotaxonomic and molecular analysis, strain E3^T represents a novel species of the genus Paenibacillus , for which the name Paenibacillus arachidis sp. nov. is proposed. The type strain is E3^T (=KCTC 33574^T=LMG 28417^T).