Arora, Amit ; Kaur, Harleen ; Wengel, Jesper ; Maiti, Souvik (2008) Effect of Locked Nucleic Acid (LNA) modification on hybridization kinetics of DNA duplex Nucleic Acids Symposium Series, 52 (1). pp. 417-418. ISSN 0261-3166
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Official URL: https://academic.oup.com/nass/article-abstract/52/...
Related URL: http://dx.doi.org/10.1093/nass/nrn212
Abstract
The effect of locked nucleic acid (LNA) modification on hybridization kinetics of DNA duplex formation has been studied at varying salt concentration (Na+ and Mg2+) using surface-plasmon resonance. The study suggested that the increased stability of LNA containing duplexes mainly originates from the slower dissociation rates constants of the duplexes. An increase in salt concentration increased the binding affinity of the individual duplexes by raising their association rate constants. Monitoring the change in binding affinity with respect to salt concentration revealed that of LNA-associated enhancement in helical stability mainly results from the changes in the non-electrostatic interactions upon duplex formation.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Oxford University Press. |
| ID Code: | 103636 |
| Deposited On: | 04 Apr 2017 06:17 |
| Last Modified: | 04 Apr 2017 06:17 |
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