Bhat, Khalid Hussain ; Srivastava, Shruti ; Kotturu, Sandeep Kumar ; Ghosh, Sudip ; Mukhopadhyay, Sangita (2017) The PPE2 protein of Mycobacterium tuberculosis translocates to host nucleus and inhibits nitric oxide production Scientific Reports, 7 . Article ID 39706. ISSN 2045-2322
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Official URL: http://www.nature.com/articles/srep39706
Related URL: http://dx.doi.org/10.1038/srep39706
Abstract
Mycobacterium tuberculosis, the bacterium that causes tuberculosis, is one of the most successful pathogens of humans. It has evolved several adaptive skills and evasion mechanisms to hijack the immunologically educated host to suit its intracellular lifestyle. Here, we show that one of the unique PPE family member proteins of M. tuberculosis, PPE2, can limit Nitric Oxide (NO) production by inhibiting inos gene transcription. PPE2 protein has a leucine zipper DNA-binding motif and a functional nuclear localization signal. PPE2 was translocated into the macrophage nucleus via the classical importin α/β pathway where it interacted with a GATA-binding site overlapping with the TATA box of inos promoter and inhibited NO production. PPE2 prolonged intracellular survival of a surrogate bacterium M. smegmatis in vitro as well as in vivo. This information is likely to improve our knowledge of host-pathogen interactions during M. tuberculosis infection which is crucial for designing effective anti-TB therapeutics.
Item Type: | Article |
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Source: | Copyright of this article belongs to Nature Publishing Group. |
ID Code: | 103535 |
Deposited On: | 09 Mar 2018 10:51 |
Last Modified: | 09 Mar 2018 10:51 |
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