Rajagopal, R. ; Sivakumar, S. ; Agrawal, N. ; Malhotra, P. ; Bhatnagar, R. K. (2002) Silencing of midgut aminopeptidase N of Spodoptera litura by double-stranded RNA establishes its role as Bacillus thuringiensis toxin receptor Journal of Biological Chemistry, 277 (49). pp. 46849-46851. ISSN 0021-9258
|
PDF
- Other
84kB |
Official URL: http://www.jbc.org/content/277/49/46849
Related URL: http://dx.doi.org/10.1074/jbc.C200523200
Abstract
Insecticidal crystal proteins of Bacillus thuringiensis bind to receptors in the midgut of susceptible insects leading to pore formation and death of the insect. The identity of the receptor is not clearly established. Recently a direct interaction between a cloned and heterologously expressed aminopeptidase (slapn) from Spodoptera litura and the Cry1C protein was demonstrated by immunofluorescence and in vitro ligand blot interaction. Here we show that administration of slapn double-stranded RNA to S. litura larvae reduces its expression. As a consequence of the reduced expression, a corresponding decrease in the sensitivity of these larvae to Cry1C toxin was observed. The gene silencing was retained during the insect's moulting and development and transmitted to the subsequent generation albeit with a reduced effect. These results directly implicate larval midgut aminopeptidase N as receptor for Bacillus thuringiensis insecticidal proteins.
Item Type: | Article |
---|---|
Source: | Copyright of this article belongs to American Society for Biochemistry and Molecular Biology. |
ID Code: | 103475 |
Deposited On: | 01 Feb 2018 17:39 |
Last Modified: | 01 Feb 2018 17:39 |
Repository Staff Only: item control page