Unique structural modulation of a non-native substrate by cochaperone DnaJ

Tiwari, Satyam ; Kumar, Vignesh ; Jayaraj, Gopal Gunanathan ; Maiti, Souvik ; Mapa, Koyeli (2013) Unique structural modulation of a non-native substrate by cochaperone DnaJ Biochemistry, 52 (6). pp. 1011-1018. ISSN 0006-2960

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Official URL: http://pubs.acs.org/doi/abs/10.1021/bi301543g

Related URL: http://dx.doi.org/10.1021/bi301543g

Abstract

The role of bacterial DnaJ protein as a cochaperone of DnaK is strongly appreciated. Although DnaJ unaccompanied by DnaK can bind unfolded as well as native substrate proteins, its role as an individual chaperone remains elusive. In this study, we demonstrate that DnaJ binds a model non-native substrate with a low nanomolar dissociation constant and, more importantly, modulates the structure of its non-native state. The structural modulation achieved by DnaJ is different compared to that achieved by the DnaK–DnaJ complex. The nature of structural modulation exerted by DnaJ is suggestive of a unique unfolding activity on the non-native substrate by the chaperone. Furthermore, we demonstrate that the zinc binding motif along with the C-terminal substrate binding domain of DnaJ is necessary and sufficient for binding and the subsequent binding-induced structural alterations of the non-native substrate. We hypothesize that this hitherto unknown structural alteration of non-native states by DnaJ might be important for its chaperoning activity by removing kinetic traps of the folding intermediates.

Item Type:Article
Source:Copyright of this article belongs to American Chemical Society.
ID Code:103374
Deposited On:02 Feb 2018 03:46
Last Modified:02 Feb 2018 03:46

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