Sharma, Shweta ; Jadli, Mohit ; Singh, Anu ; Arora, Kavita ; Malhotra, Pawan (2014) A secretory multifunctional serine protease, DegP ofPlasmodium falciparum, plays an important role in thermo-oxidative stress, parasite growth and development FEBS Journal, 281 (6). pp. 1679-1699. ISSN 1742-464X
Full text not available from this repository.
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/febs.12...
Related URL: http://dx.doi.org/10.1111/febs.12732
Abstract
Plasmodium falciparum heat shock proteins and proteases are known for their indispensable roles in parasite virulence and survival in the host cell. They neutralize various host-derived stress responses that are deleterious for parasite growth and invasion. We report identification and functional characterization of the first DegP from an apicomplexan (P. falciparum). To determine the molecular identity and functions of the parasite-encoded DegP, we complemented the Escherichia coli degP null mutant with a putative PfdegP gene, and the results showed that PfDegP complements the growth defect of the temperature sensitive DegP-deficient mutant and imparts resistance to non-permissive temperatures and oxidative stress. Molecular interaction studies showed that PfDegP exists as a complex with parasite-encoded heat shock protein 70, iron superoxide dismutase and enolase. DegP expression is significantly induced in parasite culture upon heat shock/oxidative stress. Our data suggest that the PfDegP protein may play a role in the growth and development of P. falciparum through its ability to confer protection against thermal/oxidative stress. Antibody against DegP showed anti-plasmodial activity against blood-stage parasites in vitro, suggesting that PfDegP and its associated complex may be a potential focus for new anti-malarial therapies.
Item Type: | Article |
---|---|
Source: | Copyright of this article belongs to John Wiley and Sons, Inc. |
ID Code: | 103127 |
Deposited On: | 01 Feb 2018 17:28 |
Last Modified: | 01 Feb 2018 17:28 |
Repository Staff Only: item control page