Host ICAMs play a role in cell invasion by Mycobacterium tuberculosis and Plasmodium falciparum

Bhalla, Kuhulika ; Chugh, Monika ; Mehrotra, Sonali ; Rathore, Sumit ; Tousif, Sultan ; Prakash Dwivedi, Ved ; Prakash, Prem ; Kumar Samuchiwal, Sachin ; Kumar, Sushil ; Kumar Singh, Dhiraj ; Ghanwat, Swapnil ; Kumar, Dhiraj ; Das, Gobardhan ; Mohmmed, Asif ; Malhotra, Pawan ; Ranganathan, Anand (2015) Host ICAMs play a role in cell invasion by Mycobacterium tuberculosis and Plasmodium falciparum Nature Communications, 6 . Article ID 6049. ISSN 2041-1723

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Official URL: http://www.nature.com/articles/ncomms7049

Related URL: http://dx.doi.org/10.1038/ncomms7049

Abstract

Intercellular adhesion molecules (ICAMs) belong to the immunoglobulin superfamily and participate in diverse cellular processes including host-pathogen interactions. ICAM-1 is expressed on various cell types including macrophages, whereas ICAM-4 is restricted to red blood cells. Here we report the identification of an 11-kDa synthetic protein, M5, that binds to human ICAM-1 and ICAM-4, as shown by in vitro interaction studies, surface plasmon resonance and immunolocalization. M5 greatly inhibits the invasion of macrophages and erythrocytes by Mycobacterium tuberculosis and Plasmodium falciparum, respectively. Pharmacological and siRNA-mediated inhibition of ICAM-1 expression also results in reduced M. tuberculosis invasion of macrophages. ICAM-4 binds to P. falciparum merozoites, and the addition of recombinant ICAM-4 to parasite cultures blocks invasion of erythrocytes by newly released merozoites. Our results indicate that ICAM-1 and ICAM-4 play roles in host cell invasion by M. tuberculosis and P. falciparum, respectively, either as receptors or as crucial accessory molecules.

Item Type:Article
Source:Copyright of this article belongs to Nature Publishing Group.
ID Code:103103
Deposited On:01 Feb 2018 17:27
Last Modified:01 Feb 2018 17:27

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