De novo transcriptome assembly and analysis of Sf21 cells using illumina paired end sequencing

Kakumani, Pavan Kumar ; Shukla, Rohit ; Todur, Vivek N. ; Malhotra, Pawan ; Mukherjee, Sunil K. ; Bhatnagar, Raj K. (2015) De novo transcriptome assembly and analysis of Sf21 cells using illumina paired end sequencing Biology Direct, 10 . Article ID 44-7 pages. ISSN 1745-6150

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Official URL: http://biologydirect.biomedcentral.com/articles/10...

Related URL: http://dx.doi.org/10.1186/s13062-015-0072-7

Abstract

Spodoptera is an important polyphagous agricultural insect pest in the tropical world. The genomic details are limited to understand the pest biology at molecular level. In the present study, we sequenced and assembled the transcriptome from Sf21 cells into a non redundant set of 24,038 contigs of ~ 47.38 Mb in size. A total of 26,390 unigenes were identified from the assembled transcripts and their annotation revealed the prevalent protein domains in Sf21 cells. The present study would provide a resource for gene discovery and development of functional molecular markers to understand the biology of S. frugiperda.

Item Type:Article
Source:Copyright of this article belongs to BioMed Central.
Keywords:Army Worm; Insect; Pest; Sf21 Cells; Spodoptera Frugiperda
ID Code:103092
Deposited On:01 Feb 2018 17:26
Last Modified:01 Feb 2018 17:26

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