Melatonin elevates intracellular free calcium in human platelets by inositol 1,4,5-trisphosphate independent mechanism

Kumari, Sharda ; Dash, Debabrata (2011) Melatonin elevates intracellular free calcium in human platelets by inositol 1,4,5-trisphosphate independent mechanism FEBS Letters, 585 (14). pp. 2345-2351. ISSN 0014-5793

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Official URL: http://onlinelibrary.wiley.com/doi/10.1016/j.febsl...

Related URL: http://dx.doi.org/10.1016/j.febslet.2011.05.067

Abstract

Several studies have indicated the existence of direct effects of melatonin on platelets. Here we show that, melatonin at high concentration is capable of significantly raising platelet intracellular calcium even in the absence of an agonist. The effect of melatonin on platelets was abolished by luzindole, a melatonin receptor blocker, and rotenone, while it was unaffected by cell-permeable antagonists of either inositol 1,4,5-trisphosphate (IP3) receptor, phospholipase C (PLC), or bafilomycin A1, which discharges acidic calcium stores. Melatonin-induced manganese entry provided evidence for activation of bivalent cation entry. Thus, our data suggest that melatonin evoked the elevation of platelet intracellular calcium through depletion of mitochondrial Ca2+ stores and store-operated calcium entry (SOCE), while the action was independent of the PLC-IP3 axis.

Item Type:Article
Source:Copyright of this article belongs to Wiley.
Keywords:Melatonin; Intracellular Calcium; Platelets; SERCA; SOCE
ID Code:101249
Deposited On:04 Feb 2017 17:21
Last Modified:04 Feb 2017 17:21

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