Degradation of 4-nitrophenol, 2-chloro-4-nitrophenol, and 2,4-dinitrophenol by Rhodococcus imtechensis strain RKJ300

Ghosh, Anuradha ; Khurana, Meenu ; Chauhan, Archana ; Takeo, Masahiro ; Chakraborti, Asit K. ; Jain, Rakesh K. (2010) Degradation of 4-nitrophenol, 2-chloro-4-nitrophenol, and 2,4-dinitrophenol by Rhodococcus imtechensis strain RKJ300 Environmental Science & Technology, 44 (3). pp. 1069-1077. ISSN 0013-936X

Full text not available from this repository.

Official URL: http://pubs.acs.org/doi/abs/10.1021/es9034123

Related URL: http://dx.doi.org/10.1021/es9034123

Abstract

A bacterial strain Rhodococcus imtechensis RKJ300 (= MTCC 7085T = JCM 13270T) was isolated from pesticide-contaminated soil of Punjab by the enrichment technique on minimal medium containing 4-nitrophenol. Strain RKJ300 is capable of utilizing 4-nitrophenol, 2-chloro-4-nitrophenol, and 2,4-dinitrophenol as sole sources of carbon and energy. The strain involved both oxidative and reductive catabolic mechanisms for initial transformation of these compounds. In the case of 2-chloro-4-nitrophenol, colorimetric analysis indicated that nitrite release was followed by stoichiometric elimination of chloride ions. Experiments using whole cells and cell-free extracts showed chlorohydroquinone and hydroquinone as the intermediates of 2-chloro-4-nitrophenol degradation. This is the first report of degradation on 2-chloro-4-nitrophenol by a bacterium under aerobic condition to the best of our knowledge. However, pathways for degradation of 4-nitrophenol and 2,4-dinitrophenol were similar to those reported in other strains of Rhodococcus. Laboratory-scale soil microcosm studies demonstrated that the organism was capable of degrading a mixture of nitrophenols simultaneously, indicating its applicability toward in situ bioremediation of contaminated sites. The fate of the augmented strain as monitored by the plate-counting method and hybridization technique was found to be fairly stable throughout the period of microcosm experiments.

Item Type:Article
Source:Copyright of this article belongs to American Chemical Society.
ID Code:100394
Deposited On:20 Jan 2017 08:36
Last Modified:20 Jan 2017 08:36

Repository Staff Only: item control page