The calcium binding protein EhCaBP6 is a microtubular-end binding protein in Entamoeba histolytica

Grewal, Jaspreet Singh ; Padhan, Narendra ; Aslam, Saima ; Bhattacharya, Alok ; Lohia, Anuradha (2013) The calcium binding protein EhCaBP6 is a microtubular-end binding protein in Entamoeba histolytica Cellular Microbiology, 15 (12). pp. 2020-2033. ISSN 1462-5814

Full text not available from this repository.

Official URL: http://onlinelibrary.wiley.com/doi/10.1111/cmi.121...

Related URL: http://dx.doi.org/10.1111/cmi.12167

Abstract

The genome of Entamoeba histolytica encodes several calcium binding proteins and those characterized thus far have been shown to participate predominantly in phagocytosis and endocytosis. Our study showed that EhCaBP6 has two EF-hand domains EFI and EFIII; it can bind Ca Ca 2+ in vitro and undergoes conformational transition on binding Ca 2+ suggesting that it can serve as a calcium signal sensor. EhCaBP6 is localized in the nucleus, cytoplasm and plasma membrane and is sensitive to heat stress. Unlike other Ca 2+ binding proteins that have been studied in E. histolytica, EhCaBP6 is found at microtubule ends and at the intercellular bridge with the microtubules during cytokinesis. Furthermore, increased expression of EhCaBP6 was correlated with a significant increase in the number of microtubular structures suggesting that this protein may regulate chromosome segregation and cytokinesis in E. histolytica.

Item Type:Article
Source:Copyright of this article belongs to Wiley.
ID Code:99845
Deposited On:01 Dec 2016 11:48
Last Modified:01 Dec 2016 11:48

Repository Staff Only: item control page