Episodes of horizontal gene-transfer and gene-fusion led to co-existence of different metal-ion specific glyoxalase I

Kaur, Charanpreet ; Vishnoi, Anchal ; Ariyadasa, Thilini Udayangani ; Bhattacharya, Alok ; Singla-Pareek, Sneh Lata ; Sopory, Sudhir Kumar (2013) Episodes of horizontal gene-transfer and gene-fusion led to co-existence of different metal-ion specific glyoxalase I Scientific Reports, 3 . Article ID 3076, 10 pages. ISSN 2045-2322

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Official URL: http://www.nature.com/articles/srep03076

Related URL: http://dx.doi.org/10.1038/srep03076

Abstract

Glyoxalase pathway plays an important role in stress adaptation and many clinical disorders. The first enzyme of this pathway, glyoxalase I (GlxI), uses methylglyoxal as a substrate and requires either Ni(II)/Co(II) or Zn(II) for activity. Here we have investigated the origin of different metal ion specificities of GlxI and subsequent pattern of inheritance during evolution. Our results suggest a primitive origin of single-domain Ni dependent GlxI [Ni-GlxI]. This subsequently evolved into Zn activated GlxI [Zn-GlxI] in deltaproteobacteria. However, origin of eukaryotic Zn-GlxI is different and can be traced to GlxI from Candidatus pelagibacter and Sphingomonas. In eukaryotes GlxI has evolved as two-domain protein but the corresponding Zn form is lost in plants/higher eukaryotes. In plants gene expansion has given rise to multiple two-domain Ni-GlxI which are differentially regulated under abiotic stress conditions. Our results suggest that different forms of GlxI have evolved to help plants adapt to stress.

Item Type:Article
Source:Copyright of this article belongs to Nature Publishing Group.
ID Code:99716
Deposited On:01 Dec 2016 10:22
Last Modified:01 Dec 2016 10:22

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