miR-22 regulates expression of oncogenic neuro-epithelial transforming gene 1, NET1

Ahmad, Hafiz M. ; Muiwo, Pamchui ; Ramachandran, Suganthi S. ; Pandey, Priyatama ; Gupta, Yogendra K. ; Kumar, Lalit ; Kulshreshtha, Ritu ; Bhattacharya, Alok (2014) miR-22 regulates expression of oncogenic neuro-epithelial transforming gene 1, NET1 The FEBS Journal, 281 (17). pp. 3904-3919. ISSN 1742-464X

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/febs.12...

Related URL: http://dx.doi.org/10.1111/febs.12926


MicroRNAs control cellular processes by regulating expression of their target genes. Here we report that neuro-epithelial transforming gene 1 (NET1) is a target of tumor suppressor microRNA 22 (miR-22). miR-22 is downregulated in peripheral blood mononuclear cells derived from chronic myeloid leukemia (CML) patients and in CML cell line K562. NET1 was identified as one of the targets of miR-22 using both in vitro and in vivo experiments. Either mutations or naturally occurring single-nucleotide polymorphisms in NET1 3′-UTR that map at the miR-22 binding site were found to affect binding of miR-22 to NET1 mRNA. Over expression of NET1 in K562 cells resulted in increased proliferation. However decreased proliferation and alteration in cell cycle were observed on either overexpression of miR-22 or knockdown of NET1 expression respectively. We also found that overexpression of miR-22 or NET1 knockdown inhibits actin fiber formation, probably by downregulation of NET1 as NET1 knockdown also resulted in depletion of actin fiber formation. We suggest that the oncogenic properties of CML cells are probably due to deregulated expression of NET1 as a result of altered expression of miR-22.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:CD33+; CML; HL60; K562; miR-22; NET1
ID Code:99602
Deposited On:01 Dec 2016 11:51
Last Modified:01 Dec 2016 11:51

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