Gallic acid prevents nonsteroidal anti-inflammatory drug-induced gastropathy in rat by blocking oxidative stress and apoptosis

Pal, Chinmay ; Bindu, Samik ; Dey, Sumanta ; Alam, Athar ; Goyal, Manish ; Iqbal, Mohd. Shameel ; Maity, Pallab ; Adhikari, Susanta S. ; Bandyopadhyay, Uday (2010) Gallic acid prevents nonsteroidal anti-inflammatory drug-induced gastropathy in rat by blocking oxidative stress and apoptosis Free Radical Biology & Medicine, 49 (2). pp. 258-267. ISSN 0891-5849

Full text not available from this repository.

Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.freeradbiomed.2010.04.013

Abstract

Nonsteroidal anti-inflammatory drug (NSAID)-induced oxidative stress plays a critical role in gastric mucosal cell apoptosis and gastropathy. NSAIDs induce the generation of hydroxyl radical (OH) through the release of free iron, which plays an important role in developing gastropathy. Thus, molecules having both iron-chelating and antiapoptotic properties will be beneficial in preventing NSAID-induced gastropathy. Gallic acid (GA), a polyphenolic natural product, has the capacity to chelate free iron. Here, we report that GA significantly prevents, as well as heals, NSAID-induced gastropathy. In vivo, GA blocks NSAID-mediated mitochondrial oxidative stress by preventing mitochondrial protein carbonyl formation, lipid peroxidation, and thiol depletion. In vitro, GA scavenges free radicals and blocks •OH-mediated oxidative damage. GA also attenuates gastric mucosal cell apoptosis in vivo as well as in vitro in cultured gastric mucosal cells as evident from the TUNEL assay. GA prevents NSAID-induced activation of caspase-9, a marker for the mitochondrial pathway of apoptosis, and restores NSAID-mediated collapse of the mitochondrial transmembrane potential and dehydrogenase activity. Thus, the inhibition of mitochondrial oxidative stress by GA is associated with the inhibition of NSAID-induced mitochondrial dysfunction and activation of apoptosis in gastric mucosal cells, which are responsible for gastric injury or gastropathy.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Gallic Acid; Free Radical; Oxidative Stress; Gastropathy; Apoptosis; NSAID; Reactive Oxygen Species
ID Code:99572
Deposited On:25 Nov 2016 06:51
Last Modified:25 Nov 2016 06:53

Repository Staff Only: item control page