Involvement of the α1-adrenoceptor in sleep–waking and sleep loss-induced anxiety behavior in zebrafish

Singh, A. ; Subhashini, N. ; Sharma, S. ; Mallick, B. N. (2013) Involvement of the α1-adrenoceptor in sleep–waking and sleep loss-induced anxiety behavior in zebrafish Neuroscience, 245 . pp. 136-147. ISSN 0306-4522

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Sleep is a universal phenomenon in vertebrates, and its loss affects various behaviors. Independent studies have reported that sleep loss increases anxiety; however, the detailed mechanism is unknown. Because sleep deprivation increases noradrenalin (NA), which modulates many behaviors and induces patho-physiological changes, this study utilized zebrafish as a model to investigate whether sleep loss-induced increased anxiety is modulated by NA. Continuous behavioral quiescence for at least 6 s was considered to represent sleep in zebrafish; although some authors termed it as a sleep-like state, in this study we have termed it as sleep. The activity of fish that signified sleep–waking was recorded in light–dark, during continuous dark and light; the latter induced sleep loss in fish. The latency, number of entries, time spent and distance travelled in the light chamber were assessed in a light–dark box test to estimate the anxiety behavior of normal, sleep-deprived and prazosin (PRZ)-treated fish. Zebrafish showed increased waking during light and complete loss of sleep upon continuous exposure to light for 24 h. PRZ significantly increased sleep in normal fish. Sleep-deprived fish showed an increased preference for dark (expression of increased anxiety), and this effect was prevented by PRZ, which increased sleep as well. Our findings suggest that sleep loss-induced anxiety-like behavior in zebrafish is likely to be mediated by NA’s action on the α1-adrenoceptor.

Item Type:Article
Source:Copyright of this article belongs to International Brain Research Organization.
Keywords:Anxiety; Noradrenalin; Place Preference; Prazosin; Rhythmicity; Sleep Deprivation
ID Code:99375
Deposited On:21 Apr 2016 06:09
Last Modified:21 Apr 2016 06:09

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