Differential staining of glia and neurons by modified Golgi-Cox method

Ranjan, Amit ; Mallick, Birendra Nath (2012) Differential staining of glia and neurons by modified Golgi-Cox method Journal of Neuroscience Methods, 209 (2). pp. 269-279. ISSN 0165-0270

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.jneumeth.2012.06.023


Golgi-Cox staining has been extensively used in neuroanatomical studies. Despite its unique property to impregnate and show neuronal and glial morphology, its capricious nature, less consistency and long staining time constrain users to use it as first choice. Here we report a modification of the Golgi-Cox method to reliably and differentially stain neurons and glia in the rat brain within 48 h. In summary, exposure (at any stage) of the brain or the tissue sample to be stained to a fixative [4% paraformaldehyde (PF) or 4% formaldehyde] dramatically increased the number of stained glia; whereas if the tissues were not treated with a fixative, neurons were stained. To our knowledge this is the first report of differential staining of glia and neurons using Golgi-Cox method; however, mechanism is unclear.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Differential Staining of Neurons and Glia; Fixative; Golgi-Cox Stain; Incubation Temperature
ID Code:99373
Deposited On:21 Apr 2016 07:02
Last Modified:21 Apr 2016 07:02

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