Rapid eye movement sleep deprivation modulates synapsinI expression in rat brain

Singh, Sudhuman ; Amar, Megha ; Mallick, Birendra N. (2012) Rapid eye movement sleep deprivation modulates synapsinI expression in rat brain Neuroscience Letters, 520 (1). pp. 62-66. ISSN 0304-3940

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.neulet.2012.05.031


Rapid eye movement sleep (REMS) deprivation (REMSD) has been reported to elevate neurotransmitter level in the brain; however, intracellular mechanism of its increased release was not studied. Phosphorylation of synapsinI, a synaptic vesicle-associated protein, is involved in the regulation of neurotransmitter release. In this study, rats were REMS deprived by classical flowerpot method; free moving control (FMC), large platform control (LPC) and recovery control (REC) was carried out. In another set REMS deprived rats were intraperitoneally (i.p.) injected with α1-adrenoceptor antagonist, prazosin (PRZ). Effects of REMSD on Na-K ATPase activity and on the total synapsinI as well as phosphorylated synapsinI levels were estimated in synaptosomes prepared from whole brain. It was observed that REMSD significantly increased synaptosomal Na-K ATPase activity, which was prevented by PRZ. Western blotting of the same samples by anti-synapsinI and anti-synapsinI-phosphoSer603 showed that REMSD increased both the total as well as phospho-form of synapsinI as compared to respective levels in FMC and LPC samples. These findings suggest a functional link between REMSD and synaptic vesicular mobilization at the presynaptic terminal, a process that is essential for neurotransmitter release. The findings help explaining the intracellular mechanism of elevated neurotransmitter release associated to REMSD.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Na-K ATPase; Noradrenalin; REMSD; SynapsinI-phosphoSer603; Synaptic Vesicle; Total SynapsinI
ID Code:99372
Deposited On:21 Apr 2016 08:40
Last Modified:21 Apr 2016 08:40

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