Ghosh, Saptarshi ; Kundu, Pronab ; Chattopadhyay, Nitin (2016) DNA induced sequestration of a bioactive cationic fluorophore from the lipid environment: a spectroscopic investigation Journal of Photochemistry and Photobiology B: Biology, 154 . pp. 118-125. ISSN 1011-1344
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.jphotobiol.2015.11.001
Abstract
The effect of calf-thymus DNA (ctDNA) on the lipid bound probe, formed by the cationic phenazinium dye phenosafranin (PSF) and the anionic lipid dimyristoyl-L-α-phosphatidylglycerol (DMPG), has been unearthed exploiting various spectroscopic techniques. Steady state and time-resolved fluorometric studies and measurements of circular dichroism and DNA helix melting temperature reveal that in the presence of DNA the probe is dislodged from the lipid environment and gets intercalated within the DNA helix. The work qualitatively illustrates that the anionic lipid can be used as a potential nanocarrier for delivering the cationic drugs to the most relevant biomacromolecular target, DNA.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Phenosafanin; Lipid; ctDNA; Fluorescence; Circular Dichroism; Helix Melting |
| ID Code: | 99245 |
| Deposited On: | 28 Jan 2016 05:32 |
| Last Modified: | 28 Jan 2016 05:32 |
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